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Purification of mouse axoplasmic proteins from dorsal root ganglia nerves for proteomics analysis
The study of neuronal signaling ex vivo requires the identification of the proteins that are represented within the neuronal axoplasm. Here, we describe a detailed protocol to isolate the axoplasm of peripheral and central axonal branches of sciatic dorsal root ganglia neurons in mice. The axoplasm...
Autores principales: | Kong, Guiping, Zhou, Luming, Freiwald, Anja, Shkura, Kirill, Di Giovanni, Simone |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076968/ https://www.ncbi.nlm.nih.gov/pubmed/35535163 http://dx.doi.org/10.1016/j.xpro.2022.101166 |
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