The diagnosis of hepatic fibrosis by magnetic resonance and near-infrared imaging using dual-modality nanoparticles
Hepatic fibrosis (HF), as the only reversible process of chronic liver disease, remains a big diagnostic challenge. Development of noninvasive and effective methods to assess quantitatively early-stage HF is of great clinical importance. Compared with conventional diagnostic methods, near-infrared f...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078292/ https://www.ncbi.nlm.nih.gov/pubmed/35540380 http://dx.doi.org/10.1039/c7ra10847h |
_version_ | 1784702297575522304 |
---|---|
author | Li, Yunfang Shang, Wenting Liang, Xiaoyuan Zeng, Chaoting Liu, Mingming Wang, Sudan Li, Hongjun Tian, Jie |
author_facet | Li, Yunfang Shang, Wenting Liang, Xiaoyuan Zeng, Chaoting Liu, Mingming Wang, Sudan Li, Hongjun Tian, Jie |
author_sort | Li, Yunfang |
collection | PubMed |
description | Hepatic fibrosis (HF), as the only reversible process of chronic liver disease, remains a big diagnostic challenge. Development of noninvasive and effective methods to assess quantitatively early-stage HF is of great clinical importance. Compared with conventional diagnostic methods, near-infrared fluorescence imaging (NIR) and magnetic resonance imaging (MRI) could offer highly sensitive and spatial resolution signals for HF detection. However, precise detection using contrast agents is not possible. Superparamagnetic iron oxide (SPIO) nanoparticles have low toxicity, high sensitivity and excellent biocompatibility. Integration of Fe(3)O(4) nanoparticles and indocyanine green (ICG), coupled with targeting ligand of integrin α(v)β(3), arginine–glycine–aspartic acid (RGD) expressed on hepatic stellate cells (HSCs), were used to detect HF. Both in vivo and in vitro results showed that the SPIO@SiO(2)–ICG–RGD had high stability and low cytotoxicity. The biodistribution of SPIO@SiO(2)–ICG–RGD was significantly different between mice with HF and healthy controls. SPIO@SiO(2)–ICG–RGD was characterized and the results of imaging in vitro and in vivo demonstrated the expression of integrin α(v)β(3) on activated HSCs. These data suggest that our SPIO@SiO(2)–ICG–RGD probe could be used for the diagnosis of early-stage HF. This new nanoprobe with a dual-modality imaging approach holds great potential for the diagnosis and classification of HF. |
format | Online Article Text |
id | pubmed-9078292 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90782922022-05-09 The diagnosis of hepatic fibrosis by magnetic resonance and near-infrared imaging using dual-modality nanoparticles Li, Yunfang Shang, Wenting Liang, Xiaoyuan Zeng, Chaoting Liu, Mingming Wang, Sudan Li, Hongjun Tian, Jie RSC Adv Chemistry Hepatic fibrosis (HF), as the only reversible process of chronic liver disease, remains a big diagnostic challenge. Development of noninvasive and effective methods to assess quantitatively early-stage HF is of great clinical importance. Compared with conventional diagnostic methods, near-infrared fluorescence imaging (NIR) and magnetic resonance imaging (MRI) could offer highly sensitive and spatial resolution signals for HF detection. However, precise detection using contrast agents is not possible. Superparamagnetic iron oxide (SPIO) nanoparticles have low toxicity, high sensitivity and excellent biocompatibility. Integration of Fe(3)O(4) nanoparticles and indocyanine green (ICG), coupled with targeting ligand of integrin α(v)β(3), arginine–glycine–aspartic acid (RGD) expressed on hepatic stellate cells (HSCs), were used to detect HF. Both in vivo and in vitro results showed that the SPIO@SiO(2)–ICG–RGD had high stability and low cytotoxicity. The biodistribution of SPIO@SiO(2)–ICG–RGD was significantly different between mice with HF and healthy controls. SPIO@SiO(2)–ICG–RGD was characterized and the results of imaging in vitro and in vivo demonstrated the expression of integrin α(v)β(3) on activated HSCs. These data suggest that our SPIO@SiO(2)–ICG–RGD probe could be used for the diagnosis of early-stage HF. This new nanoprobe with a dual-modality imaging approach holds great potential for the diagnosis and classification of HF. The Royal Society of Chemistry 2018-02-12 /pmc/articles/PMC9078292/ /pubmed/35540380 http://dx.doi.org/10.1039/c7ra10847h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Li, Yunfang Shang, Wenting Liang, Xiaoyuan Zeng, Chaoting Liu, Mingming Wang, Sudan Li, Hongjun Tian, Jie The diagnosis of hepatic fibrosis by magnetic resonance and near-infrared imaging using dual-modality nanoparticles |
title | The diagnosis of hepatic fibrosis by magnetic resonance and near-infrared imaging using dual-modality nanoparticles |
title_full | The diagnosis of hepatic fibrosis by magnetic resonance and near-infrared imaging using dual-modality nanoparticles |
title_fullStr | The diagnosis of hepatic fibrosis by magnetic resonance and near-infrared imaging using dual-modality nanoparticles |
title_full_unstemmed | The diagnosis of hepatic fibrosis by magnetic resonance and near-infrared imaging using dual-modality nanoparticles |
title_short | The diagnosis of hepatic fibrosis by magnetic resonance and near-infrared imaging using dual-modality nanoparticles |
title_sort | diagnosis of hepatic fibrosis by magnetic resonance and near-infrared imaging using dual-modality nanoparticles |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078292/ https://www.ncbi.nlm.nih.gov/pubmed/35540380 http://dx.doi.org/10.1039/c7ra10847h |
work_keys_str_mv | AT liyunfang thediagnosisofhepaticfibrosisbymagneticresonanceandnearinfraredimagingusingdualmodalitynanoparticles AT shangwenting thediagnosisofhepaticfibrosisbymagneticresonanceandnearinfraredimagingusingdualmodalitynanoparticles AT liangxiaoyuan thediagnosisofhepaticfibrosisbymagneticresonanceandnearinfraredimagingusingdualmodalitynanoparticles AT zengchaoting thediagnosisofhepaticfibrosisbymagneticresonanceandnearinfraredimagingusingdualmodalitynanoparticles AT liumingming thediagnosisofhepaticfibrosisbymagneticresonanceandnearinfraredimagingusingdualmodalitynanoparticles AT wangsudan thediagnosisofhepaticfibrosisbymagneticresonanceandnearinfraredimagingusingdualmodalitynanoparticles AT lihongjun thediagnosisofhepaticfibrosisbymagneticresonanceandnearinfraredimagingusingdualmodalitynanoparticles AT tianjie thediagnosisofhepaticfibrosisbymagneticresonanceandnearinfraredimagingusingdualmodalitynanoparticles AT liyunfang diagnosisofhepaticfibrosisbymagneticresonanceandnearinfraredimagingusingdualmodalitynanoparticles AT shangwenting diagnosisofhepaticfibrosisbymagneticresonanceandnearinfraredimagingusingdualmodalitynanoparticles AT liangxiaoyuan diagnosisofhepaticfibrosisbymagneticresonanceandnearinfraredimagingusingdualmodalitynanoparticles AT zengchaoting diagnosisofhepaticfibrosisbymagneticresonanceandnearinfraredimagingusingdualmodalitynanoparticles AT liumingming diagnosisofhepaticfibrosisbymagneticresonanceandnearinfraredimagingusingdualmodalitynanoparticles AT wangsudan diagnosisofhepaticfibrosisbymagneticresonanceandnearinfraredimagingusingdualmodalitynanoparticles AT lihongjun diagnosisofhepaticfibrosisbymagneticresonanceandnearinfraredimagingusingdualmodalitynanoparticles AT tianjie diagnosisofhepaticfibrosisbymagneticresonanceandnearinfraredimagingusingdualmodalitynanoparticles |