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An impedimetric determination of alkaline phosphatase activity based on the oxidation reaction mediated by Cu(2+) bound to poly-thymine DNA
We herein describe a novel impedimetric method to determine alkaline phosphatase (ALP) activity based on the Cu(2+)-mediated oxidation of ascorbic acid on a specific DNA probe-modified electrode. In this method, pyrophosphate (PPi) capable of complexing with Cu(2+) is employed as a substrate of the...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078965/ https://www.ncbi.nlm.nih.gov/pubmed/35541507 http://dx.doi.org/10.1039/c7ra13642k |