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An impedimetric determination of alkaline phosphatase activity based on the oxidation reaction mediated by Cu(2+) bound to poly-thymine DNA

We herein describe a novel impedimetric method to determine alkaline phosphatase (ALP) activity based on the Cu(2+)-mediated oxidation of ascorbic acid on a specific DNA probe-modified electrode. In this method, pyrophosphate (PPi) capable of complexing with Cu(2+) is employed as a substrate of the...

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Detalles Bibliográficos
Autores principales: Lee, Joon Young, Ahn, Jun Ki, Park, Ki Soo, Park, Hyun Gyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078965/
https://www.ncbi.nlm.nih.gov/pubmed/35541507
http://dx.doi.org/10.1039/c7ra13642k