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Purification, structure and anti-oxidation of polysaccharides from the fruit of Nitraria tangutorum Bobr.

In this paper, polysaccharides were extracted from the fruits of Nitraria tangutorum Bobr. (NTWP) using a hot water extraction method and extraction conditions were optimized by RSM. The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water...

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Detalles Bibliográficos
Autores principales: Zhao, Baotang, Liu, Jing, Chen, Xin, Zhang, Ji, Wang, Junlong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079049/
https://www.ncbi.nlm.nih.gov/pubmed/35542817
http://dx.doi.org/10.1039/c8ra01125g
Descripción
Sumario:In this paper, polysaccharides were extracted from the fruits of Nitraria tangutorum Bobr. (NTWP) using a hot water extraction method and extraction conditions were optimized by RSM. The optimal conditions were determined as follows: extraction time 7 h, extraction temperature 60 °C, ratio of water to raw material 15 : 1, and with these conditions, the yield was 14.01 ± 0.11%. After purification using DEAE-cellulose column and Sephadex G-200 column, NTWP-II was successfully obtained. The results of GC-MS and SEC-LLS analysis suggested that monosaccharide composition of NTWP-II was composed of Rha, Ara, Man, Glc and Gal with the molar ratio of 1.14 : 2.5 : 3.00 : 2.69 : 5.28 and M(w), M(w)/M(n) and R(z) 2.29 × 10(5), 1.32, 15.22. The detailed structure of NTWP-II was characterized by FT-IR, NMR. Based on these analyses, the structure of the repeating unit of NTWP-II was established.