Protocol to quantify polyphosphate in human cell lines using a tagged PPBD peptide

Polyphosphate (polyP) is an evolutionarily conserved polymer of phosphates that is difficult to study in human cells because of its low concentration and high lability. First, we described how to express and purify Xpress-tagged PPBD (Ppx1 PolyP Binding Domain). We describe the detection and quantif...

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Detalles Bibliográficos
Autores principales: Jiménez, Javier, Lázaro, Blanca, Sarrias, Ana, Tadeo, Francisco J., Pérez-Montero, Marta, Clotet, Josep, Bru, Samuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079103/
https://www.ncbi.nlm.nih.gov/pubmed/35542178
http://dx.doi.org/10.1016/j.xpro.2022.101363

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079103/
https://www.ncbi.nlm.nih.gov/pubmed/35542178
http://dx.doi.org/10.1016/j.xpro.2022.101363

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