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Protocol to quantify polyphosphate in human cell lines using a tagged PPBD peptide
Polyphosphate (polyP) is an evolutionarily conserved polymer of phosphates that is difficult to study in human cells because of its low concentration and high lability. First, we described how to express and purify Xpress-tagged PPBD (Ppx1 PolyP Binding Domain). We describe the detection and quantif...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079103/ https://www.ncbi.nlm.nih.gov/pubmed/35542178 http://dx.doi.org/10.1016/j.xpro.2022.101363 |