Horseradish peroxidase-mediated synthesis of an antioxidant gallic acid-g-chitosan derivative and its preservation application in cherry tomatoes

Owing to their good solubility and film-forming properties, phenolic acid-g-chitosan derivatives can be used for preservation of fruits and vegetables. However, the chemical synthesis used for the preparation of these derivatives poses a great challenge to food safety. In this study, a method involv...

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Autores principales: Zhang, Xiao, Wu, Hao, Zhang, Linan, Sun, Qingjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080819/
https://www.ncbi.nlm.nih.gov/pubmed/35541661
http://dx.doi.org/10.1039/c8ra02632g
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author Zhang, Xiao
Wu, Hao
Zhang, Linan
Sun, Qingjie
author_facet Zhang, Xiao
Wu, Hao
Zhang, Linan
Sun, Qingjie
author_sort Zhang, Xiao
collection PubMed
description Owing to their good solubility and film-forming properties, phenolic acid-g-chitosan derivatives can be used for preservation of fruits and vegetables. However, the chemical synthesis used for the preparation of these derivatives poses a great challenge to food safety. In this study, a method involving horseradish peroxidase catalysis was used to prepare a gallic acid-g-chitosan derivative. The grafting mechanism was studied. Then, the derivative's ability to scavenge free radicals and its preserving application in cherry tomatoes were evaluated. The results indicated that the reaction for horseradish peroxidase catalysis occurred between the amino group of chitosan and the carboxyl group of gallic acid. After enzymatic grafting, the gallic acid-g-chitosan derivative possessed excellent antioxidant abilities in scavenging DPPH, hydroxyl, and superoxide anion radicals. When the derivative was used for the preservation of cherry tomatoes, the results showed that it could effectively protect the ascorbate–glutathione cycle and antioxidant enzyme system of cherry tomatoes and inhibit enzymatic browning. In addition, since this derivative delayed the postharvest senescence of cherry tomatoes, the aroma compounds remain relatively constant throughout the storage period.
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spelling pubmed-90808192022-05-09 Horseradish peroxidase-mediated synthesis of an antioxidant gallic acid-g-chitosan derivative and its preservation application in cherry tomatoes Zhang, Xiao Wu, Hao Zhang, Linan Sun, Qingjie RSC Adv Chemistry Owing to their good solubility and film-forming properties, phenolic acid-g-chitosan derivatives can be used for preservation of fruits and vegetables. However, the chemical synthesis used for the preparation of these derivatives poses a great challenge to food safety. In this study, a method involving horseradish peroxidase catalysis was used to prepare a gallic acid-g-chitosan derivative. The grafting mechanism was studied. Then, the derivative's ability to scavenge free radicals and its preserving application in cherry tomatoes were evaluated. The results indicated that the reaction for horseradish peroxidase catalysis occurred between the amino group of chitosan and the carboxyl group of gallic acid. After enzymatic grafting, the gallic acid-g-chitosan derivative possessed excellent antioxidant abilities in scavenging DPPH, hydroxyl, and superoxide anion radicals. When the derivative was used for the preservation of cherry tomatoes, the results showed that it could effectively protect the ascorbate–glutathione cycle and antioxidant enzyme system of cherry tomatoes and inhibit enzymatic browning. In addition, since this derivative delayed the postharvest senescence of cherry tomatoes, the aroma compounds remain relatively constant throughout the storage period. The Royal Society of Chemistry 2018-06-04 /pmc/articles/PMC9080819/ /pubmed/35541661 http://dx.doi.org/10.1039/c8ra02632g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Zhang, Xiao
Wu, Hao
Zhang, Linan
Sun, Qingjie
Horseradish peroxidase-mediated synthesis of an antioxidant gallic acid-g-chitosan derivative and its preservation application in cherry tomatoes
title Horseradish peroxidase-mediated synthesis of an antioxidant gallic acid-g-chitosan derivative and its preservation application in cherry tomatoes
title_full Horseradish peroxidase-mediated synthesis of an antioxidant gallic acid-g-chitosan derivative and its preservation application in cherry tomatoes
title_fullStr Horseradish peroxidase-mediated synthesis of an antioxidant gallic acid-g-chitosan derivative and its preservation application in cherry tomatoes
title_full_unstemmed Horseradish peroxidase-mediated synthesis of an antioxidant gallic acid-g-chitosan derivative and its preservation application in cherry tomatoes
title_short Horseradish peroxidase-mediated synthesis of an antioxidant gallic acid-g-chitosan derivative and its preservation application in cherry tomatoes
title_sort horseradish peroxidase-mediated synthesis of an antioxidant gallic acid-g-chitosan derivative and its preservation application in cherry tomatoes
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080819/
https://www.ncbi.nlm.nih.gov/pubmed/35541661
http://dx.doi.org/10.1039/c8ra02632g
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