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The structure and configuration changes of multifunctional peptide vectors enhance gene delivery efficiency

We designed a series of peptide vectors that contain functional fragments with the goal of enhancing cellular internalization and gene transfection efficiency. The functional fragments included a cell-penetrating peptide (R(9)), a cationic amphiphilic α-helical peptide [(LLKK)(3)-H(6) or (LLHH)(3)],...

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Detalles Bibliográficos
Autores principales: Yang, Sen, Meng, Zhao, Kang, Ziyao, Sun, Chao, Wang, Taoran, Feng, Siliang, Meng, Qingbin, Liu, Keliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9084241/
https://www.ncbi.nlm.nih.gov/pubmed/35542475
http://dx.doi.org/10.1039/c8ra04101f
Descripción
Sumario:We designed a series of peptide vectors that contain functional fragments with the goal of enhancing cellular internalization and gene transfection efficiency. The functional fragments included a cell-penetrating peptide (R(9)), a cationic amphiphilic α-helical peptide [(LLKK)(3)-H(6) or (LLHH)(3)], a stearyl moiety, and cysteine residues. Vectors were also synthesized with D-type amino acids to improve their proteolytic stability. The conformations, particle sizes, and zeta potentials for complexes of these peptides with pGL3 plasmid DNA were characterized by circular dichroism and dynamic light scattering. In addition, cellular uptake of the peptide/DNA complexes and gene transfection efficiency were investigated with fluorescence-activated cell sorting and confocal laser-scanning microscopy. Greater transfection efficiency was achieved with the vectors containing the R(9) segment, and the efficiency was greater than Lipo2000. In addition, the D-type C(18)-c(llkk)(3)ch(6)-r(9) had about 7 times and 5.5 times the transfection efficiency of Lipo2000 in 293T cells and NIH-3T3 cells at the N/P ratio of 6, respectively. Overall, the multifunctional peptide gene vectors containing the R(9) segment exhibited enhanced cellular internalization, a high gene transfection efficiency, and low cytotoxicity.