No-ozone cold plasma can kill oral pathogenic microbes in H(2)O(2)-dependent and independent manner

To apply the sterilisation effect of low-temperature plasma to the oral cavity, the issue of ozone from plasma must be addressed. In this study, a new technology for generating cold plasma with almost no ozone is developed and is named Nozone (no-ozone) Cold Plasma (NCP) technology. The antimicrobia...

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Autores principales: Park, Nam-Sook, Yun, Se-Eun, Lee, Hyun-Young, Lee, Hae June, Choi, Jeong-Hae, Kim, Gyoo-Cheon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085805/
https://www.ncbi.nlm.nih.gov/pubmed/35534525
http://dx.doi.org/10.1038/s41598-022-11665-z
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author Park, Nam-Sook
Yun, Se-Eun
Lee, Hyun-Young
Lee, Hae June
Choi, Jeong-Hae
Kim, Gyoo-Cheon
author_facet Park, Nam-Sook
Yun, Se-Eun
Lee, Hyun-Young
Lee, Hae June
Choi, Jeong-Hae
Kim, Gyoo-Cheon
author_sort Park, Nam-Sook
collection PubMed
description To apply the sterilisation effect of low-temperature plasma to the oral cavity, the issue of ozone from plasma must be addressed. In this study, a new technology for generating cold plasma with almost no ozone is developed and is named Nozone (no-ozone) Cold Plasma (NCP) technology. The antimicrobial efficacy of the NCP against four oral pathogens is tested, and its specific mechanism is elucidated. The treatment of NCP on oral pathogenic microbes on a solid medium generated a growth inhibition zone. When NCP is applied to oral pathogens in a liquid medium, the growth of microbes decreased by more than 10(5) colony forming units, and the bactericidal effect of NCP remained after the installation of dental tips. The bactericidal effect of NCP in the liquid medium is due to the increase in hydrogen peroxide levels in the medium. However, the bactericidal effect of NCP in the solid medium depends on the charged elements of the NCP. Furthermore, the surface bactericidal efficiency of the dental-tip-installed NCP is proportional to the pore size of the tips and inversely proportional to the length of the tips. Overall, we expect this NCP device to be widely used in dentistry in the near future.
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spelling pubmed-90858052022-05-11 No-ozone cold plasma can kill oral pathogenic microbes in H(2)O(2)-dependent and independent manner Park, Nam-Sook Yun, Se-Eun Lee, Hyun-Young Lee, Hae June Choi, Jeong-Hae Kim, Gyoo-Cheon Sci Rep Article To apply the sterilisation effect of low-temperature plasma to the oral cavity, the issue of ozone from plasma must be addressed. In this study, a new technology for generating cold plasma with almost no ozone is developed and is named Nozone (no-ozone) Cold Plasma (NCP) technology. The antimicrobial efficacy of the NCP against four oral pathogens is tested, and its specific mechanism is elucidated. The treatment of NCP on oral pathogenic microbes on a solid medium generated a growth inhibition zone. When NCP is applied to oral pathogens in a liquid medium, the growth of microbes decreased by more than 10(5) colony forming units, and the bactericidal effect of NCP remained after the installation of dental tips. The bactericidal effect of NCP in the liquid medium is due to the increase in hydrogen peroxide levels in the medium. However, the bactericidal effect of NCP in the solid medium depends on the charged elements of the NCP. Furthermore, the surface bactericidal efficiency of the dental-tip-installed NCP is proportional to the pore size of the tips and inversely proportional to the length of the tips. Overall, we expect this NCP device to be widely used in dentistry in the near future. Nature Publishing Group UK 2022-05-09 /pmc/articles/PMC9085805/ /pubmed/35534525 http://dx.doi.org/10.1038/s41598-022-11665-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Park, Nam-Sook
Yun, Se-Eun
Lee, Hyun-Young
Lee, Hae June
Choi, Jeong-Hae
Kim, Gyoo-Cheon
No-ozone cold plasma can kill oral pathogenic microbes in H(2)O(2)-dependent and independent manner
title No-ozone cold plasma can kill oral pathogenic microbes in H(2)O(2)-dependent and independent manner
title_full No-ozone cold plasma can kill oral pathogenic microbes in H(2)O(2)-dependent and independent manner
title_fullStr No-ozone cold plasma can kill oral pathogenic microbes in H(2)O(2)-dependent and independent manner
title_full_unstemmed No-ozone cold plasma can kill oral pathogenic microbes in H(2)O(2)-dependent and independent manner
title_short No-ozone cold plasma can kill oral pathogenic microbes in H(2)O(2)-dependent and independent manner
title_sort no-ozone cold plasma can kill oral pathogenic microbes in h(2)o(2)-dependent and independent manner
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085805/
https://www.ncbi.nlm.nih.gov/pubmed/35534525
http://dx.doi.org/10.1038/s41598-022-11665-z
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