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Analysis of Key Genes Responsible for Low Urea Production in Saccharomyces cerevisiae JH301

There is a potential safety risk with ethyl carbamate (EC) in Hongqu Huangjiu production; 90% of the EC in rice wine is produced by the reaction of the urea with the alcohol of Saccharomyces cerevisiae. In our previous experiments, we screened and obtained a S. cerevisiae strain JH301 that offered l...

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Autores principales: Liang, Zhangcheng, Su, Hao, Ren, Xiangyun, Lin, Xiaozi, He, Zhigang, Li, Xiangyou, Zheng, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9087593/
https://www.ncbi.nlm.nih.gov/pubmed/35558109
http://dx.doi.org/10.3389/fmicb.2022.894661
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author Liang, Zhangcheng
Su, Hao
Ren, Xiangyun
Lin, Xiaozi
He, Zhigang
Li, Xiangyou
Zheng, Yan
author_facet Liang, Zhangcheng
Su, Hao
Ren, Xiangyun
Lin, Xiaozi
He, Zhigang
Li, Xiangyou
Zheng, Yan
author_sort Liang, Zhangcheng
collection PubMed
description There is a potential safety risk with ethyl carbamate (EC) in Hongqu Huangjiu production; 90% of the EC in rice wine is produced by the reaction of the urea with the alcohol of Saccharomyces cerevisiae. In our previous experiments, we screened and obtained a S. cerevisiae strain JH301 that offered low urea production. However, the key genes responsible for low urea production of strain JH301 remain unclear. Here, the whole genome sequencing of S. cerevisiae strain JH301 was accomplished via a next-generation high-throughput sequencing and long-read sequencing technology. There are six main pathways related to the urea metabolism of strain JH301 based on KEGG pathway mapping. Three species-specific genes are related to the urea metabolism pathways and were found in comparative genome analysis between strains JH301 and S288c during Hongqu Huangjiu production for the first time. Finally, the ARG80 gene was found to be likely a key gene responsible for low urea production of S. cerevisiae strain JH301, as determined by PCR and qRT-PCR check analyses from DNA and RNA levers. In conclusion, the results are useful for a scientific understanding of the mechanism of low urea production by Saccharomyces cerevisiae during Hongqu Huangjiu fermentation. It also is important to control the urea and EC contents in Hongqu Huangjiu production.
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spelling pubmed-90875932022-05-11 Analysis of Key Genes Responsible for Low Urea Production in Saccharomyces cerevisiae JH301 Liang, Zhangcheng Su, Hao Ren, Xiangyun Lin, Xiaozi He, Zhigang Li, Xiangyou Zheng, Yan Front Microbiol Microbiology There is a potential safety risk with ethyl carbamate (EC) in Hongqu Huangjiu production; 90% of the EC in rice wine is produced by the reaction of the urea with the alcohol of Saccharomyces cerevisiae. In our previous experiments, we screened and obtained a S. cerevisiae strain JH301 that offered low urea production. However, the key genes responsible for low urea production of strain JH301 remain unclear. Here, the whole genome sequencing of S. cerevisiae strain JH301 was accomplished via a next-generation high-throughput sequencing and long-read sequencing technology. There are six main pathways related to the urea metabolism of strain JH301 based on KEGG pathway mapping. Three species-specific genes are related to the urea metabolism pathways and were found in comparative genome analysis between strains JH301 and S288c during Hongqu Huangjiu production for the first time. Finally, the ARG80 gene was found to be likely a key gene responsible for low urea production of S. cerevisiae strain JH301, as determined by PCR and qRT-PCR check analyses from DNA and RNA levers. In conclusion, the results are useful for a scientific understanding of the mechanism of low urea production by Saccharomyces cerevisiae during Hongqu Huangjiu fermentation. It also is important to control the urea and EC contents in Hongqu Huangjiu production. Frontiers Media S.A. 2022-04-26 /pmc/articles/PMC9087593/ /pubmed/35558109 http://dx.doi.org/10.3389/fmicb.2022.894661 Text en Copyright © 2022 Liang, Su, Ren, Lin, He, Li and Zheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liang, Zhangcheng
Su, Hao
Ren, Xiangyun
Lin, Xiaozi
He, Zhigang
Li, Xiangyou
Zheng, Yan
Analysis of Key Genes Responsible for Low Urea Production in Saccharomyces cerevisiae JH301
title Analysis of Key Genes Responsible for Low Urea Production in Saccharomyces cerevisiae JH301
title_full Analysis of Key Genes Responsible for Low Urea Production in Saccharomyces cerevisiae JH301
title_fullStr Analysis of Key Genes Responsible for Low Urea Production in Saccharomyces cerevisiae JH301
title_full_unstemmed Analysis of Key Genes Responsible for Low Urea Production in Saccharomyces cerevisiae JH301
title_short Analysis of Key Genes Responsible for Low Urea Production in Saccharomyces cerevisiae JH301
title_sort analysis of key genes responsible for low urea production in saccharomyces cerevisiae jh301
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9087593/
https://www.ncbi.nlm.nih.gov/pubmed/35558109
http://dx.doi.org/10.3389/fmicb.2022.894661
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