Cargando…

Identifying New Ligands for JNK3 by Fluorescence Thermal Shift Assays and Native Mass Spectrometry

[Image: see text] The c-Jun N-terminal kinases (JNKs) are evolutionary highly conserved serine/threonine kinases. Numerous findings suggest that JNK3 is involved in the pathogenesis of neurodegenerative diseases, so the inhibition of JNK3 may be a potential therapeutic intervention. The identificati...

Descripción completa

Detalles Bibliográficos
Autores principales:	Cheng, Chongyun, Liu, Miaomiao, Gao, Xiaoqin, Wu, Dong, Pu, Mengchen, Ma, Jun, Quinn, Ronald J., Xiao, Zhicheng, Liu, Zhijie
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2022
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9088906/ https://www.ncbi.nlm.nih.gov/pubmed/35559183 http://dx.doi.org/10.1021/acsomega.2c00340

Ejemplares similares

Development of a target identification approach using native mass spectrometry
por: Liu, Miaomiao, et al.
Publicado: (2021)

POT1 stability and binding measured by fluorescence thermal shift assays
por: DeLeeuw, Lynn W., et al.
Publicado: (2021)

Collision-Induced Affinity Selection Mass Spectrometry for Identification of Ligands
por: Mak, Tin, et al.
Publicado: (2022)

EDTA aggregates induce SYPRO orange-based fluorescence in thermal shift assay
por: Kroeger, Tobias, et al.
Publicado: (2017)

Native Mass Spectrometry for the Study of PROTAC GNE‐987‐Containing Ternary Complexes
por: Sternicki, Louise M., et al.
Publicado: (2021)

Protein–ligand interactions investigated by thermal shift assays (TSA) and dual polarization interferometry (DPI)
por: Grøftehauge, Morten K., et al.
Publicado: (2015)

Protocol to assess receptor-ligand binding in C. elegans using adapted thermal shift assays
por: Tse, Samantha Y., et al.
Publicado: (2023)

Native Mass Spectrometry in Fragment-Based Drug Discovery
por: Pedro, Liliana, et al.
Publicado: (2016)

A Fluorescence-Based Thermal Shift Assay Identifies Inhibitors of Mitogen Activated Protein Kinase Kinase 4
por: Krishna, Sankar N., et al.
Publicado: (2013)

Looking for protein stabilizing drugs with thermal shift assay
por: Andreotti, Giuseppina, et al.
Publicado: (2015)

Correction: A Fluorescence-Based Thermal Shift Assay Identifies Inhibitors of Mitogen Activated Protein Kinase Kinase 4
por: Krishna, Sankar N., et al.
Publicado: (2013)

Nanoscale Ion Emitters in Native Mass Spectrometry for Measuring Ligand–Protein Binding Affinities
por: Nguyen, Giang T. H., et al.
Publicado: (2019)

Optimization of protein samples for NMR using thermal shift assays
por: Kozak, Sandra, et al.
Publicado: (2016)

Homogeneous Assay for Target Engagement Utilizing Bioluminescent Thermal Shift
por: Dart, Melanie L., et al.
Publicado: (2018)

A superior loading control for the cellular thermal shift assay
por: Delport, Alexandré, et al.
Publicado: (2022)

Detection of thermal shift in cellular Keap1 by protein-protein interaction inhibitors using immunoblot- and fluorescence microplate-based assays
por: Dayalan Naidu, Sharadha, et al.
Publicado: (2022)

Mass spectrometry of intact membrane proteins: shifting towards a more native-like context
por: Oluwole, Abraham, et al.
Publicado: (2023)

Development of a quantitative fluorescence-based ligand-binding assay
por: Breen, Conor J., et al.
Publicado: (2016)

Discovery of a Natural Product That Binds to the Mycobacterium tuberculosis Protein Rv1466 Using Native Mass Spectrometry
por: Elnaas, Ali R., et al.
Publicado: (2020)

Thermal Shift Assay for Small GTPase Stability Screening: Evaluation and Suitability
por: Kopra, Kari, et al.
Publicado: (2022)

Real-Time Cellular Thermal Shift Assay to Monitor Target Engagement
por: Sanchez, Tino W., et al.
Publicado: (2022)

A Simple Fluorescence Affinity Assay to Decipher Uranyl‐Binding to Native Proteins
por: Laporte, Fanny, et al.
Publicado: (2022)

Characterization of ligand binding to dopamine receptors with fluorescence anisotropy based assay
por: Allikalt, Anni, et al.
Publicado: (2015)

Bead based facile assay for sensitive quantification of native state green fluorescent protein
por: Kim, Jung Min, et al.
Publicado: (2020)

A fluorescence-based helicase assay: application to the screening of G-quadruplex ligands
por: Mendoza, Oscar, et al.
Publicado: (2015)

A kinetic fluorescence polarization ligand assay for monitoring BAX early activation
por: Gelles, Jesse D., et al.
Publicado: (2022)

Native Mass Spectrometry: What is in the Name?
por: Leney, Aneika C., et al.
Publicado: (2016)

High-Resolution Native Mass Spectrometry
por: Tamara, Sem, et al.
Publicado: (2021)

Detection of Chemical Engagement of Solute Carrier Proteins by a Cellular Thermal Shift Assay
por: Hashimoto, Mari, et al.
Publicado: (2018)

Development of NMR and thermal shift assays for the evaluation of Mycobacterium tuberculosis isocitrate lyase inhibitors
por: Bhusal, Ram Prasad, et al.
Publicado: (2017)

Label-free target identification using in-gel fluorescence difference via thermal stability shift
por: Park, Hankum, et al.
Publicado: (2017)

High-Throughput Interrogation of Ligand Binding Mode Using a Fluorescence-Based Assay**
por: Śledź, Paweł, et al.
Publicado: (2012)

A fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin
por: Menchon, Grégory, et al.
Publicado: (2018)

Experimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays
por: Phaneuf, Clifford G., et al.
Publicado: (2023)

TSA-CRAFT: A Free Software for Automatic and Robust Thermal Shift Assay Data Analysis
por: Lee, Po-Hsien, et al.
Publicado: (2019)

Parsimonious Charge Deconvolution for Native Mass Spectrometry
por: Bern, Marshall, et al.
Publicado: (2018)

Mass spectrometry-based ligand binding assays on adenosine A(1) and A(2A) receptors
por: Massink, A., et al.
Publicado: (2015)

Halogenated Thermally Activated Delayed Fluorescence Materials for Efficient Scintillation
por: Wang, Xiao, et al.
Publicado: (2023)

Thermal Dissociation Assay for Time-Resolved Fluorescence Detection of Protein Post-Translational Modifications
por: Eskonen, Ville, et al.
Publicado: (2019)

Suppression of ischemia in arterial occlusive disease by JNK-promoted native collateral artery development
por: Ramo, Kasmir, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_o66hk4hk5b5ivbrj2u6jcfl1en