Enhancing the thermostability of Rhizopus oryzae lipase by combined mutation of hot-spots and engineering a disulfide bond

Rhizopus oryzae lipase (ROL) is important because of its extreme sn-1,3-regioselectivity, but it shows poor thermostability, which severely restricts its application. In this work, the thermostability of ROL was greatly enhanced by rational design. First, several sites that may affect the thermostab...

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Detalles Bibliográficos
Autores principales: Zhao, Jiong-feng, Wang, Zhe, Gao, Fei-long, Lin, Jian-ping, Yang, Li-rong, Wu, Mian-bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091645/
https://www.ncbi.nlm.nih.gov/pubmed/35559271
http://dx.doi.org/10.1039/c8ra07767c

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9091645/
https://www.ncbi.nlm.nih.gov/pubmed/35559271
http://dx.doi.org/10.1039/c8ra07767c

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