Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials

With the increasing use of mass cytometry in clinical research, a simplified and standardized protocol for immunophenotyping human peripheral blood mononuclear cells (PBMCs) in clinical trials is needed. We present a simplified in-plate staining protocol for up to 80 samples, for laboratories of all...

Descripción completa

Detalles Bibliográficos
Autores principales: Abrecht, Charlotte, Hallisey, Margaret, Dennis, Jenna, Nazzaro, Matthew, Brainard, Martha, Hathaway, Emma, Schork, Abigail N., Hodi, F. Stephen, Severgnini, Mariano, Baginska, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092992/
https://www.ncbi.nlm.nih.gov/pubmed/35573480
http://dx.doi.org/10.1016/j.xpro.2022.101362

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092992/
https://www.ncbi.nlm.nih.gov/pubmed/35573480
http://dx.doi.org/10.1016/j.xpro.2022.101362

Ejemplares similares