Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials

With the increasing use of mass cytometry in clinical research, a simplified and standardized protocol for immunophenotyping human peripheral blood mononuclear cells (PBMCs) in clinical trials is needed. We present a simplified in-plate staining protocol for up to 80 samples, for laboratories of all...

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Detalles Bibliográficos
Autores principales: Abrecht, Charlotte, Hallisey, Margaret, Dennis, Jenna, Nazzaro, Matthew, Brainard, Martha, Hathaway, Emma, Schork, Abigail N., Hodi, F. Stephen, Severgnini, Mariano, Baginska, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092992/
https://www.ncbi.nlm.nih.gov/pubmed/35573480
http://dx.doi.org/10.1016/j.xpro.2022.101362
Descripción
Sumario:With the increasing use of mass cytometry in clinical research, a simplified and standardized protocol for immunophenotyping human peripheral blood mononuclear cells (PBMCs) in clinical trials is needed. We present a simplified in-plate staining protocol for up to 80 samples, for laboratories of all mass cytometry expertise levels, aimed to generate reproducible datasets for large clinical cohorts. In this protocol, we provide details on the requirements to obtain meaningful results, spanning from sample quality, barcoding, and batch-freezing of stained samples.

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