Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials

With the increasing use of mass cytometry in clinical research, a simplified and standardized protocol for immunophenotyping human peripheral blood mononuclear cells (PBMCs) in clinical trials is needed. We present a simplified in-plate staining protocol for up to 80 samples, for laboratories of all...

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Autores principales: Abrecht, Charlotte, Hallisey, Margaret, Dennis, Jenna, Nazzaro, Matthew, Brainard, Martha, Hathaway, Emma, Schork, Abigail N., Hodi, F. Stephen, Severgnini, Mariano, Baginska, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092992/
https://www.ncbi.nlm.nih.gov/pubmed/35573480
http://dx.doi.org/10.1016/j.xpro.2022.101362
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author Abrecht, Charlotte
Hallisey, Margaret
Dennis, Jenna
Nazzaro, Matthew
Brainard, Martha
Hathaway, Emma
Schork, Abigail N.
Hodi, F. Stephen
Severgnini, Mariano
Baginska, Joanna
author_facet Abrecht, Charlotte
Hallisey, Margaret
Dennis, Jenna
Nazzaro, Matthew
Brainard, Martha
Hathaway, Emma
Schork, Abigail N.
Hodi, F. Stephen
Severgnini, Mariano
Baginska, Joanna
author_sort Abrecht, Charlotte
collection PubMed
description With the increasing use of mass cytometry in clinical research, a simplified and standardized protocol for immunophenotyping human peripheral blood mononuclear cells (PBMCs) in clinical trials is needed. We present a simplified in-plate staining protocol for up to 80 samples, for laboratories of all mass cytometry expertise levels, aimed to generate reproducible datasets for large clinical cohorts. In this protocol, we provide details on the requirements to obtain meaningful results, spanning from sample quality, barcoding, and batch-freezing of stained samples.
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spelling pubmed-90929922022-05-12 Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials Abrecht, Charlotte Hallisey, Margaret Dennis, Jenna Nazzaro, Matthew Brainard, Martha Hathaway, Emma Schork, Abigail N. Hodi, F. Stephen Severgnini, Mariano Baginska, Joanna STAR Protoc Protocol With the increasing use of mass cytometry in clinical research, a simplified and standardized protocol for immunophenotyping human peripheral blood mononuclear cells (PBMCs) in clinical trials is needed. We present a simplified in-plate staining protocol for up to 80 samples, for laboratories of all mass cytometry expertise levels, aimed to generate reproducible datasets for large clinical cohorts. In this protocol, we provide details on the requirements to obtain meaningful results, spanning from sample quality, barcoding, and batch-freezing of stained samples. Elsevier 2022-05-05 /pmc/articles/PMC9092992/ /pubmed/35573480 http://dx.doi.org/10.1016/j.xpro.2022.101362 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Abrecht, Charlotte
Hallisey, Margaret
Dennis, Jenna
Nazzaro, Matthew
Brainard, Martha
Hathaway, Emma
Schork, Abigail N.
Hodi, F. Stephen
Severgnini, Mariano
Baginska, Joanna
Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials
title Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials
title_full Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials
title_fullStr Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials
title_full_unstemmed Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials
title_short Simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human PBMC samples in clinical trials
title_sort simplified mass cytometry protocol for in-plate staining, barcoding, and cryopreservation of human pbmc samples in clinical trials
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9092992/
https://www.ncbi.nlm.nih.gov/pubmed/35573480
http://dx.doi.org/10.1016/j.xpro.2022.101362
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