An Optimized Tissue Dissociation Protocol for Single-Cell RNA Sequencing Analysis of Fresh and Cultured Human Skin Biopsies

We present an optimized dissociation protocol for preparing high-quality skin cell suspensions for in-depth single-cell RNA-sequencing (scRNA-seq) analysis of fresh and cultured human skin. Our protocol enabled the isolation of a consistently high number of highly viable skin cells from small freshl...

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Detalles Bibliográficos
Autores principales: Burja, Blaž, Paul, Dominique, Tastanova, Aizhan, Edalat, Sam G., Gerber, Reto, Houtman, Miranda, Elhai, Muriel, Bürki, Kristina, Staeger, Ramon, Restivo, Gaetana, Lang, Ramon, Sodin-Semrl, Snezna, Lakota, Katja, Tomšič, Matija, Levesque, Mitchell P., Distler, Oliver, Rotar, Žiga, Robinson, Mark D., Frank-Bertoncelj, Mojca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Cell and Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096112/
https://www.ncbi.nlm.nih.gov/pubmed/35573685
http://dx.doi.org/10.3389/fcell.2022.872688

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9096112/
https://www.ncbi.nlm.nih.gov/pubmed/35573685
http://dx.doi.org/10.3389/fcell.2022.872688

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