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A CHO-Based Cell-Free Dual Fluorescence Reporter System for the Straightforward Assessment of Amber Suppression and scFv Functionality

Incorporation of noncanonical amino acids (ncAAs) with bioorthogonal reactive groups by amber suppression allows the generation of synthetic proteins with desired novel properties. Such modified molecules are in high demand for basic research and therapeutic applications such as cancer treatment and...

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Detalles Bibliográficos
Autores principales: Krebs, Simon K., Rakotoarinoro, Nathanaël, Stech, Marlitt, Zemella, Anne, Kubick, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098822/
https://www.ncbi.nlm.nih.gov/pubmed/35573244
http://dx.doi.org/10.3389/fbioe.2022.873906