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A CHO-Based Cell-Free Dual Fluorescence Reporter System for the Straightforward Assessment of Amber Suppression and scFv Functionality
Incorporation of noncanonical amino acids (ncAAs) with bioorthogonal reactive groups by amber suppression allows the generation of synthetic proteins with desired novel properties. Such modified molecules are in high demand for basic research and therapeutic applications such as cancer treatment and...
Autores principales: | Krebs, Simon K., Rakotoarinoro, Nathanaël, Stech, Marlitt, Zemella, Anne, Kubick, Stefan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098822/ https://www.ncbi.nlm.nih.gov/pubmed/35573244 http://dx.doi.org/10.3389/fbioe.2022.873906 |
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