Microfluidics delivery of DARPP-32 into HeLa cells maintains viability for in-cell NMR spectroscopy

High-resolution structural studies of proteins and protein complexes in a native eukaryotic environment present a challenge to structural biology. In-cell NMR can characterize atomic resolution structures but requires high concentrations of labeled proteins in intact cells. Most exogenous delivery t...

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Detalles Bibliográficos
Autores principales: Sciolino, Nicholas, Liu, Anna, Breindel, Leonard, Burz, David S., Sulchek, Todd, Shekhtman, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098904/
https://www.ncbi.nlm.nih.gov/pubmed/35551287
http://dx.doi.org/10.1038/s42003-022-03412-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9098904/
https://www.ncbi.nlm.nih.gov/pubmed/35551287
http://dx.doi.org/10.1038/s42003-022-03412-x

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