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High-Yield Production of Catalytically Active Regulatory [NiFe]-Hydrogenase From Cupriavidus necator in Escherichia coli

Hydrogenases are biotechnologically relevant metalloenzymes that catalyze the reversible conversion of molecular hydrogen into protons and electrons. The O(2)-tolerant [NiFe]-hydrogenases from Cupriavidus necator (formerly Ralstonia eutropha) are of particular interest as they maintain catalysis eve...

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Detalles Bibliográficos
Autores principales: Fan, Qin, Caserta, Giorgio, Lorent, Christian, Zebger, Ingo, Neubauer, Peter, Lenz, Oliver, Gimpel, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9100943/
https://www.ncbi.nlm.nih.gov/pubmed/35572669
http://dx.doi.org/10.3389/fmicb.2022.894375