Having a Same Type IIS Enzyme’s Restriction Site on Guide RNA Sequence Does Not Affect Golden Gate (GG) Cloning and Subsequent CRISPR/Cas Mutagenesis

Golden gate/modular cloning facilitates faster and more efficient cloning by utilizing the unique features of the type IIS restriction enzymes. However, it is known that targeted insertion of DNA fragment(s) must not include internal type IIS restriction recognition sites. In the case of cloning CRI...

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Detalles Bibliográficos
Autores principales: Moniruzzaman, M., Zhong, Yun, Huang, Zhifeng, Zhong, Guangyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101711/
https://www.ncbi.nlm.nih.gov/pubmed/35563297
http://dx.doi.org/10.3390/ijms23094889

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9101711/
https://www.ncbi.nlm.nih.gov/pubmed/35563297
http://dx.doi.org/10.3390/ijms23094889

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