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Chromatographic Determination of the Mycotoxin Patulin in 219 Chinese Tea Samples and Implications for Human Health

Patulin (PAT) is a mycotoxin, with several acute, chronic, and cellular level toxic effects, produced by various fungi. A limit for PAT in food of has been set by authorities to guarantee food safety. Research on PAT in tea has been very limited although tea is the second largest beverage in the wor...

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Autores principales: Li, Hai, Liu, Candi, Luo, Shurong, Zhu, Sijie, Tang, Shan, Zeng, Huimei, Qin, Yu, Ma, Ming, Zeng, Dong, van Beek, Teris A., Wang, Hui, Chen, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103431/
https://www.ncbi.nlm.nih.gov/pubmed/35566203
http://dx.doi.org/10.3390/molecules27092852
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author Li, Hai
Liu, Candi
Luo, Shurong
Zhu, Sijie
Tang, Shan
Zeng, Huimei
Qin, Yu
Ma, Ming
Zeng, Dong
van Beek, Teris A.
Wang, Hui
Chen, Bo
author_facet Li, Hai
Liu, Candi
Luo, Shurong
Zhu, Sijie
Tang, Shan
Zeng, Huimei
Qin, Yu
Ma, Ming
Zeng, Dong
van Beek, Teris A.
Wang, Hui
Chen, Bo
author_sort Li, Hai
collection PubMed
description Patulin (PAT) is a mycotoxin, with several acute, chronic, and cellular level toxic effects, produced by various fungi. A limit for PAT in food of has been set by authorities to guarantee food safety. Research on PAT in tea has been very limited although tea is the second largest beverage in the world. In this paper, HPLC−DAD and GC−MS methods for analysis of PAT in different tea products, such as non-fermented (green tea), partially fermented (oolong tea, white tea, yellow tea), completely fermented (black tea), and post-fermented (dark tea and Pu-erh tea) teas were developed. The methods showed good selectivity with regard to tea pigments and 5-hydroxymethylfurfural (5-HMF) and a recovery of 90–102% for PAT at a 10–100 ppb spiking level. Limit of detection (LOD) and limit of quantification (LOQ) in tea were 1.5 ng/g and 5.0 ng/g for HPLC−UV, and 0.25 ng/g and 0.83 ng/g for GC−MS. HPLC was simpler and more robust, while GC−MS showed higher sensitivity and selectivity. GC−MS was used to validate the HPLC−UV method and prove its accuracy. The PAT content of 219 Chinese tea samples was investigated. Most tea samples contained less than 10 ng/g, ten more than 10 ng/g and two more than 50 ng/g. The results imply that tea products in China are safe with regard to their PAT content. Even an extreme daily consumption of 25 g of the tea with the highest PAT content (124 ng/g), translates to an intake of only 3 μg/person/day, which is still an order of magnitude below the maximum allowed daily intake of 30 µg for an adult.
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spelling pubmed-91034312022-05-14 Chromatographic Determination of the Mycotoxin Patulin in 219 Chinese Tea Samples and Implications for Human Health Li, Hai Liu, Candi Luo, Shurong Zhu, Sijie Tang, Shan Zeng, Huimei Qin, Yu Ma, Ming Zeng, Dong van Beek, Teris A. Wang, Hui Chen, Bo Molecules Article Patulin (PAT) is a mycotoxin, with several acute, chronic, and cellular level toxic effects, produced by various fungi. A limit for PAT in food of has been set by authorities to guarantee food safety. Research on PAT in tea has been very limited although tea is the second largest beverage in the world. In this paper, HPLC−DAD and GC−MS methods for analysis of PAT in different tea products, such as non-fermented (green tea), partially fermented (oolong tea, white tea, yellow tea), completely fermented (black tea), and post-fermented (dark tea and Pu-erh tea) teas were developed. The methods showed good selectivity with regard to tea pigments and 5-hydroxymethylfurfural (5-HMF) and a recovery of 90–102% for PAT at a 10–100 ppb spiking level. Limit of detection (LOD) and limit of quantification (LOQ) in tea were 1.5 ng/g and 5.0 ng/g for HPLC−UV, and 0.25 ng/g and 0.83 ng/g for GC−MS. HPLC was simpler and more robust, while GC−MS showed higher sensitivity and selectivity. GC−MS was used to validate the HPLC−UV method and prove its accuracy. The PAT content of 219 Chinese tea samples was investigated. Most tea samples contained less than 10 ng/g, ten more than 10 ng/g and two more than 50 ng/g. The results imply that tea products in China are safe with regard to their PAT content. Even an extreme daily consumption of 25 g of the tea with the highest PAT content (124 ng/g), translates to an intake of only 3 μg/person/day, which is still an order of magnitude below the maximum allowed daily intake of 30 µg for an adult. MDPI 2022-04-29 /pmc/articles/PMC9103431/ /pubmed/35566203 http://dx.doi.org/10.3390/molecules27092852 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Hai
Liu, Candi
Luo, Shurong
Zhu, Sijie
Tang, Shan
Zeng, Huimei
Qin, Yu
Ma, Ming
Zeng, Dong
van Beek, Teris A.
Wang, Hui
Chen, Bo
Chromatographic Determination of the Mycotoxin Patulin in 219 Chinese Tea Samples and Implications for Human Health
title Chromatographic Determination of the Mycotoxin Patulin in 219 Chinese Tea Samples and Implications for Human Health
title_full Chromatographic Determination of the Mycotoxin Patulin in 219 Chinese Tea Samples and Implications for Human Health
title_fullStr Chromatographic Determination of the Mycotoxin Patulin in 219 Chinese Tea Samples and Implications for Human Health
title_full_unstemmed Chromatographic Determination of the Mycotoxin Patulin in 219 Chinese Tea Samples and Implications for Human Health
title_short Chromatographic Determination of the Mycotoxin Patulin in 219 Chinese Tea Samples and Implications for Human Health
title_sort chromatographic determination of the mycotoxin patulin in 219 chinese tea samples and implications for human health
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9103431/
https://www.ncbi.nlm.nih.gov/pubmed/35566203
http://dx.doi.org/10.3390/molecules27092852
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