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ChipCytometry for multiplexed detection of protein and mRNA markers on human FFPE tissue samples

In this protocol, we describe the use of ChipCytometry to combine RNA in situ hybridization and antibody staining for multiplexed tissue imaging of human formalin-fixed and paraffin-embedded tissue samples. The advantages of ChipCytometry are long-term storage for re-interrogation and advanced image...

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Detalles Bibliográficos
Autores principales: Jarosch, Sebastian, Köhlen, Jan, Wagner, Sabrina, D’Ippolito, Elvira, Busch, Dirk H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9109191/
https://www.ncbi.nlm.nih.gov/pubmed/35586313
http://dx.doi.org/10.1016/j.xpro.2022.101374