Cell-line dependency in cerebral organoid induction: cautionary observations in Alzheimer’s disease patient-derived induced pluripotent stem cells

The cerebral organoid (CO) model has been used in the study of various neurodegenerative diseases owing to its physiological implications. However, the CO model may only be representative of certain clinical findings in affected patients, while some features are not recapitulated. In this study, we...

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Autores principales: Lee, Ju-Hyun, Yoo, Geon, Choi, Juhyun, Park, Si-Hyung, Shin, Hyogeun, Prasad, Renuka, Lee, Yeunehee, Ahn, Mee Ryung, Cho, Il-Joo, Sun, Woong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Micro Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9109296/
https://www.ncbi.nlm.nih.gov/pubmed/35578344
http://dx.doi.org/10.1186/s13041-022-00928-5
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author Lee, Ju-Hyun
Yoo, Geon
Choi, Juhyun
Park, Si-Hyung
Shin, Hyogeun
Prasad, Renuka
Lee, Yeunehee
Ahn, Mee Ryung
Cho, Il-Joo
Sun, Woong
author_facet Lee, Ju-Hyun
Yoo, Geon
Choi, Juhyun
Park, Si-Hyung
Shin, Hyogeun
Prasad, Renuka
Lee, Yeunehee
Ahn, Mee Ryung
Cho, Il-Joo
Sun, Woong
author_sort Lee, Ju-Hyun
collection PubMed
description The cerebral organoid (CO) model has been used in the study of various neurodegenerative diseases owing to its physiological implications. However, the CO model may only be representative of certain clinical findings in affected patients, while some features are not recapitulated. In this study, we found that neurons in the CO model from patients with Alzheimer’s disease were less responsive to depolarization, in contrast to previous reports. This difference may be partly attributed to the variations in brain spatial identity depending on the genetic background of the induced pluripotent stem cells. Our current observation raises concerns that the phenotypes observed in the CO model need to be carefully evaluated for their clinical implications. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13041-022-00928-5.
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spelling pubmed-91092962022-05-17 Cell-line dependency in cerebral organoid induction: cautionary observations in Alzheimer’s disease patient-derived induced pluripotent stem cells Lee, Ju-Hyun Yoo, Geon Choi, Juhyun Park, Si-Hyung Shin, Hyogeun Prasad, Renuka Lee, Yeunehee Ahn, Mee Ryung Cho, Il-Joo Sun, Woong Mol Brain Micro Report The cerebral organoid (CO) model has been used in the study of various neurodegenerative diseases owing to its physiological implications. However, the CO model may only be representative of certain clinical findings in affected patients, while some features are not recapitulated. In this study, we found that neurons in the CO model from patients with Alzheimer’s disease were less responsive to depolarization, in contrast to previous reports. This difference may be partly attributed to the variations in brain spatial identity depending on the genetic background of the induced pluripotent stem cells. Our current observation raises concerns that the phenotypes observed in the CO model need to be carefully evaluated for their clinical implications. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13041-022-00928-5. BioMed Central 2022-05-16 /pmc/articles/PMC9109296/ /pubmed/35578344 http://dx.doi.org/10.1186/s13041-022-00928-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Micro Report
Lee, Ju-Hyun
Yoo, Geon
Choi, Juhyun
Park, Si-Hyung
Shin, Hyogeun
Prasad, Renuka
Lee, Yeunehee
Ahn, Mee Ryung
Cho, Il-Joo
Sun, Woong
Cell-line dependency in cerebral organoid induction: cautionary observations in Alzheimer’s disease patient-derived induced pluripotent stem cells
title Cell-line dependency in cerebral organoid induction: cautionary observations in Alzheimer’s disease patient-derived induced pluripotent stem cells
title_full Cell-line dependency in cerebral organoid induction: cautionary observations in Alzheimer’s disease patient-derived induced pluripotent stem cells
title_fullStr Cell-line dependency in cerebral organoid induction: cautionary observations in Alzheimer’s disease patient-derived induced pluripotent stem cells
title_full_unstemmed Cell-line dependency in cerebral organoid induction: cautionary observations in Alzheimer’s disease patient-derived induced pluripotent stem cells
title_short Cell-line dependency in cerebral organoid induction: cautionary observations in Alzheimer’s disease patient-derived induced pluripotent stem cells
title_sort cell-line dependency in cerebral organoid induction: cautionary observations in alzheimer’s disease patient-derived induced pluripotent stem cells
topic Micro Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9109296/
https://www.ncbi.nlm.nih.gov/pubmed/35578344
http://dx.doi.org/10.1186/s13041-022-00928-5
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