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Xer recombination for the automatic deletion of selectable marker genes from plasmids in enteric bacteria

Antibiotic resistance genes are widely used to select bacteria transformed with plasmids and to prevent plasmid loss from cultures, yet antibiotics represent contaminants in the biopharmaceutical manufacturing process, and retaining antibiotic resistance genes in vaccines and biological therapies is...

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Detalles Bibliográficos
Autores principales: Salerno, Paola, Leckenby, Matthew W, Humphrey, Bruce, Cranenburgh, Rocky M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113270/
https://www.ncbi.nlm.nih.gov/pubmed/35601876
http://dx.doi.org/10.1093/synbio/ysac005