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Single-molecule study of the effects of temperature, pH, and RNA base on the stepwise enzyme kinetics of 10–23 deoxyribozyme

We investigated how the stepwise enzyme kinetics of 10–23 deoxyribozyme was affected by temperature, pH, and RNA residue of the substrate at the single-molecule level. A deoxyribozyme-substrate system was employed to temporally categorize a single-turnover reaction into four distinct steps: binding,...

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Detalles Bibliográficos
Autores principales: Jung, Jiwon, Kim, Seon Yong, Kim, Seong Keun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113834/
https://www.ncbi.nlm.nih.gov/pubmed/35702195
http://dx.doi.org/10.1039/d2ra02131e