Establishment and maintenance of thyroid organoids from human cancer cells

Here, we describe a protocol to generate organoids from human thyroid cancer cells. Starting from the same patient-derived cells, we establish both organoids and primary lines. The organoid medium is supplemented with conditioned medium obtained from the primary cell line. This modification enables...

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Detalles Bibliográficos
Autores principales: Pecce, Valeria, Sponziello, Marialuisa, Bini, Simone, Grani, Giorgio, Durante, Cosimo, Verrienti, Antonella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9115321/
https://www.ncbi.nlm.nih.gov/pubmed/35600922
http://dx.doi.org/10.1016/j.xpro.2022.101393

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9115321/
https://www.ncbi.nlm.nih.gov/pubmed/35600922
http://dx.doi.org/10.1016/j.xpro.2022.101393

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