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Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models

Coherent fluorescence imaging with two objective lenses (4Pi detection) enables single-molecule localization microscopy with sub-10 nm spatial resolution in three dimensions. Despite its outstanding sensitivity, wider application of this technique has been hindered by complex instrumentation and the...

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Detalles Bibliográficos
Autores principales: Bates, Mark, Keller-Findeisen, Jan, Przybylski, Adrian, Hüper, Andreas, Stephan, Till, Ilgen, Peter, Cereceda Delgado, Angel R., D’Este, Elisa, Egner, Alexander, Jakobs, Stefan, Sahl, Steffen J., Hell, Stefan W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9119851/
https://www.ncbi.nlm.nih.gov/pubmed/35577958
http://dx.doi.org/10.1038/s41592-022-01465-8