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NADH-independent enzymatic assay to quantify extracellular and intracellular L-lactate levels

Lactate is a central metabolite in energy metabolism and is also involved in cell signaling and epigenetic regulations. Here, we describe an NADH-independent enzymatic assay allowing rapid, selective, and sensitive quantification of L-lactate down to the pmol range. We detail lactate extraction from...

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Detalles Bibliográficos
Autores principales: Bouchez, Cyrielle L., Daubon, Thomas, Mourier, Arnaud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120226/
https://www.ncbi.nlm.nih.gov/pubmed/35600935
http://dx.doi.org/10.1016/j.xpro.2022.101403