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NADH-independent enzymatic assay to quantify extracellular and intracellular L-lactate levels
Lactate is a central metabolite in energy metabolism and is also involved in cell signaling and epigenetic regulations. Here, we describe an NADH-independent enzymatic assay allowing rapid, selective, and sensitive quantification of L-lactate down to the pmol range. We detail lactate extraction from...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120226/ https://www.ncbi.nlm.nih.gov/pubmed/35600935 http://dx.doi.org/10.1016/j.xpro.2022.101403 |