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Isolation of human and mouse myeloid-derived suppressor cells for metabolic analysis

Metabolic reprogramming is associated with myeloid-derived suppressor cell (MDSC) immunosuppressive function. Here, we outline the process for acquiring MDSCs from human and murine sources for subsequent analysis of fatty acid oxidation, oxidative phosphorylation, and glycolysis using the Seahorse X...

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Detalles Bibliográficos
Autores principales: Choi, Jee Eun, MacDonald, Cameron R., Gandhi, Nishant, Das, Gokul, Repasky, Elizabeth A., Mohammadpour, Hemn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120228/
https://www.ncbi.nlm.nih.gov/pubmed/35600927
http://dx.doi.org/10.1016/j.xpro.2022.101389