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Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics

Mammalian splenic tissue is rich in functional immune cells, primarily lymphocytes which can mask low-abundance populations in downstream analyses. This protocol enriches minority immune cell populations from mouse spleen via immunomagnetic negative depletion to generate an untouched enriched cell f...

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Detalles Bibliográficos
Autores principales: Schulze, Thomas T., Neville, Andrew J., Chapman, Ryan C., Davis, Paul H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120244/
https://www.ncbi.nlm.nih.gov/pubmed/35600930
http://dx.doi.org/10.1016/j.xpro.2022.101402