Cargando…
Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics
Mammalian splenic tissue is rich in functional immune cells, primarily lymphocytes which can mask low-abundance populations in downstream analyses. This protocol enriches minority immune cell populations from mouse spleen via immunomagnetic negative depletion to generate an untouched enriched cell f...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120244/ https://www.ncbi.nlm.nih.gov/pubmed/35600930 http://dx.doi.org/10.1016/j.xpro.2022.101402 |
_version_ | 1784710895125921792 |
---|---|
author | Schulze, Thomas T. Neville, Andrew J. Chapman, Ryan C. Davis, Paul H. |
author_facet | Schulze, Thomas T. Neville, Andrew J. Chapman, Ryan C. Davis, Paul H. |
author_sort | Schulze, Thomas T. |
collection | PubMed |
description | Mammalian splenic tissue is rich in functional immune cells, primarily lymphocytes which can mask low-abundance populations in downstream analyses. This protocol enriches minority immune cell populations from mouse spleen via immunomagnetic negative depletion to generate an untouched enriched cell fraction. Enriched cells are then spiked with untouched splenocytes in a controlled repopulation, validated by flow cytometry and results in a single-cell transcriptomic clustering analysis with a broadened cellular landscape. |
format | Online Article Text |
id | pubmed-9120244 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-91202442022-05-21 Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics Schulze, Thomas T. Neville, Andrew J. Chapman, Ryan C. Davis, Paul H. STAR Protoc Protocol Mammalian splenic tissue is rich in functional immune cells, primarily lymphocytes which can mask low-abundance populations in downstream analyses. This protocol enriches minority immune cell populations from mouse spleen via immunomagnetic negative depletion to generate an untouched enriched cell fraction. Enriched cells are then spiked with untouched splenocytes in a controlled repopulation, validated by flow cytometry and results in a single-cell transcriptomic clustering analysis with a broadened cellular landscape. Elsevier 2022-05-17 /pmc/articles/PMC9120244/ /pubmed/35600930 http://dx.doi.org/10.1016/j.xpro.2022.101402 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Schulze, Thomas T. Neville, Andrew J. Chapman, Ryan C. Davis, Paul H. Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics |
title | Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics |
title_full | Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics |
title_fullStr | Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics |
title_full_unstemmed | Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics |
title_short | Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics |
title_sort | mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120244/ https://www.ncbi.nlm.nih.gov/pubmed/35600930 http://dx.doi.org/10.1016/j.xpro.2022.101402 |
work_keys_str_mv | AT schulzethomast mousesplenocyteenrichmentstrategiesvianegativeselectionforbroadenedsinglecelltranscriptomics AT nevilleandrewj mousesplenocyteenrichmentstrategiesvianegativeselectionforbroadenedsinglecelltranscriptomics AT chapmanryanc mousesplenocyteenrichmentstrategiesvianegativeselectionforbroadenedsinglecelltranscriptomics AT davispaulh mousesplenocyteenrichmentstrategiesvianegativeselectionforbroadenedsinglecelltranscriptomics |