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Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics

Mammalian splenic tissue is rich in functional immune cells, primarily lymphocytes which can mask low-abundance populations in downstream analyses. This protocol enriches minority immune cell populations from mouse spleen via immunomagnetic negative depletion to generate an untouched enriched cell f...

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Detalles Bibliográficos
Autores principales: Schulze, Thomas T., Neville, Andrew J., Chapman, Ryan C., Davis, Paul H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120244/
https://www.ncbi.nlm.nih.gov/pubmed/35600930
http://dx.doi.org/10.1016/j.xpro.2022.101402
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author Schulze, Thomas T.
Neville, Andrew J.
Chapman, Ryan C.
Davis, Paul H.
author_facet Schulze, Thomas T.
Neville, Andrew J.
Chapman, Ryan C.
Davis, Paul H.
author_sort Schulze, Thomas T.
collection PubMed
description Mammalian splenic tissue is rich in functional immune cells, primarily lymphocytes which can mask low-abundance populations in downstream analyses. This protocol enriches minority immune cell populations from mouse spleen via immunomagnetic negative depletion to generate an untouched enriched cell fraction. Enriched cells are then spiked with untouched splenocytes in a controlled repopulation, validated by flow cytometry and results in a single-cell transcriptomic clustering analysis with a broadened cellular landscape.
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spelling pubmed-91202442022-05-21 Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics Schulze, Thomas T. Neville, Andrew J. Chapman, Ryan C. Davis, Paul H. STAR Protoc Protocol Mammalian splenic tissue is rich in functional immune cells, primarily lymphocytes which can mask low-abundance populations in downstream analyses. This protocol enriches minority immune cell populations from mouse spleen via immunomagnetic negative depletion to generate an untouched enriched cell fraction. Enriched cells are then spiked with untouched splenocytes in a controlled repopulation, validated by flow cytometry and results in a single-cell transcriptomic clustering analysis with a broadened cellular landscape. Elsevier 2022-05-17 /pmc/articles/PMC9120244/ /pubmed/35600930 http://dx.doi.org/10.1016/j.xpro.2022.101402 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Schulze, Thomas T.
Neville, Andrew J.
Chapman, Ryan C.
Davis, Paul H.
Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics
title Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics
title_full Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics
title_fullStr Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics
title_full_unstemmed Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics
title_short Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics
title_sort mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120244/
https://www.ncbi.nlm.nih.gov/pubmed/35600930
http://dx.doi.org/10.1016/j.xpro.2022.101402
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