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Mouse splenocyte enrichment strategies via negative selection for broadened single-cell transcriptomics
Mammalian splenic tissue is rich in functional immune cells, primarily lymphocytes which can mask low-abundance populations in downstream analyses. This protocol enriches minority immune cell populations from mouse spleen via immunomagnetic negative depletion to generate an untouched enriched cell f...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120244/ https://www.ncbi.nlm.nih.gov/pubmed/35600930 http://dx.doi.org/10.1016/j.xpro.2022.101402 |