Mechanics of CRISPR-Cas12a and engineered variants on λ-DNA

Cas12a is an RNA-guided endonuclease that is emerging as a powerful genome-editing tool. Here, we selected a target site on bacteriophage λ-DNA and used optical tweezers combined with fluorescence to provide mechanistic insight into wild type Cas12a and three engineered variants, where the specific...

Descripción completa

Detalles Bibliográficos
Autores principales: Paul, Bijoya, Chaubet, Loïc, Verver, Dideke Emma, Montoya, Guillermo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122593/
https://www.ncbi.nlm.nih.gov/pubmed/34951457
http://dx.doi.org/10.1093/nar/gkab1272

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122593/
https://www.ncbi.nlm.nih.gov/pubmed/34951457
http://dx.doi.org/10.1093/nar/gkab1272

Ejemplares similares