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Mechanics of CRISPR-Cas12a and engineered variants on λ-DNA

Cas12a is an RNA-guided endonuclease that is emerging as a powerful genome-editing tool. Here, we selected a target site on bacteriophage λ-DNA and used optical tweezers combined with fluorescence to provide mechanistic insight into wild type Cas12a and three engineered variants, where the specific...

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Detalles Bibliográficos
Autores principales:	Paul, Bijoya, Chaubet, Loïc, Verver, Dideke Emma, Montoya, Guillermo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122593/ https://www.ncbi.nlm.nih.gov/pubmed/34951457 http://dx.doi.org/10.1093/nar/gkab1272

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