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Noise reduction by upstream open reading frames
Gene expression is prone to burst production, making it a highly noisy process that requires additional controls. Upstream open reading frames (uORFs) are widely present in the 5′ leader sequences of 30–50% of eukaryotic messenger RNAs(1–3). The translation of uORFs can repress the translation effic...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122824/ https://www.ncbi.nlm.nih.gov/pubmed/35501454 http://dx.doi.org/10.1038/s41477-022-01136-8 |
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author | Wu, Ho-Wei Fajiculay, Erickson Wu, Jing-Fen Yan, Ching-Cher Sanders Hsu, Chao-Ping Wu, Shu-Hsing |
author_facet | Wu, Ho-Wei Fajiculay, Erickson Wu, Jing-Fen Yan, Ching-Cher Sanders Hsu, Chao-Ping Wu, Shu-Hsing |
author_sort | Wu, Ho-Wei |
collection | PubMed |
description | Gene expression is prone to burst production, making it a highly noisy process that requires additional controls. Upstream open reading frames (uORFs) are widely present in the 5′ leader sequences of 30–50% of eukaryotic messenger RNAs(1–3). The translation of uORFs can repress the translation efficiency of the downstream main coding sequences. Whether the low translation efficiency leads to a different variation, or noise, in gene expression has not been investigated, nor has the direct biological impact of uORF-repressed translation. Here we show that uORFs achieve low but precise protein production in plant cells, possibly by reducing the protein production rate. We also demonstrate that, by buffering a stable TIMING OF CAB EXPRESSION 1 (TOC1) protein production level, uORFs contribute to the robust operation of the plant circadian clock. Our results provide both an action model and the biological impact of uORFs in translational control to mitigate transcriptional noise for precise protein production. |
format | Online Article Text |
id | pubmed-9122824 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-91228242022-05-22 Noise reduction by upstream open reading frames Wu, Ho-Wei Fajiculay, Erickson Wu, Jing-Fen Yan, Ching-Cher Sanders Hsu, Chao-Ping Wu, Shu-Hsing Nat Plants Letter Gene expression is prone to burst production, making it a highly noisy process that requires additional controls. Upstream open reading frames (uORFs) are widely present in the 5′ leader sequences of 30–50% of eukaryotic messenger RNAs(1–3). The translation of uORFs can repress the translation efficiency of the downstream main coding sequences. Whether the low translation efficiency leads to a different variation, or noise, in gene expression has not been investigated, nor has the direct biological impact of uORF-repressed translation. Here we show that uORFs achieve low but precise protein production in plant cells, possibly by reducing the protein production rate. We also demonstrate that, by buffering a stable TIMING OF CAB EXPRESSION 1 (TOC1) protein production level, uORFs contribute to the robust operation of the plant circadian clock. Our results provide both an action model and the biological impact of uORFs in translational control to mitigate transcriptional noise for precise protein production. Nature Publishing Group UK 2022-05-02 2022 /pmc/articles/PMC9122824/ /pubmed/35501454 http://dx.doi.org/10.1038/s41477-022-01136-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Letter Wu, Ho-Wei Fajiculay, Erickson Wu, Jing-Fen Yan, Ching-Cher Sanders Hsu, Chao-Ping Wu, Shu-Hsing Noise reduction by upstream open reading frames |
title | Noise reduction by upstream open reading frames |
title_full | Noise reduction by upstream open reading frames |
title_fullStr | Noise reduction by upstream open reading frames |
title_full_unstemmed | Noise reduction by upstream open reading frames |
title_short | Noise reduction by upstream open reading frames |
title_sort | noise reduction by upstream open reading frames |
topic | Letter |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9122824/ https://www.ncbi.nlm.nih.gov/pubmed/35501454 http://dx.doi.org/10.1038/s41477-022-01136-8 |
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