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Single-cell suspension preparation from murine organs following in vivo mRNA-LNP exposure
We describe a protocol to study inflammatory responses triggered by the mRNA-lipid nanoparticle (LNP) vaccine formulations in skin, muscle, and lung and the adaptive immune responses induced in the draining lymph nodes. Here, we will present how to deliver these reagents through intradermal, intramu...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127192/ https://www.ncbi.nlm.nih.gov/pubmed/35620070 http://dx.doi.org/10.1016/j.xpro.2022.101350 |