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Single-cell suspension preparation from murine organs following in vivo mRNA-LNP exposure

We describe a protocol to study inflammatory responses triggered by the mRNA-lipid nanoparticle (LNP) vaccine formulations in skin, muscle, and lung and the adaptive immune responses induced in the draining lymph nodes. Here, we will present how to deliver these reagents through intradermal, intramu...

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Detalles Bibliográficos
Autores principales: Ndeupen, Sonia, Qin, Zhen, Igyártó, Botond Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127192/
https://www.ncbi.nlm.nih.gov/pubmed/35620070
http://dx.doi.org/10.1016/j.xpro.2022.101350