Single-cell suspension preparation from murine organs following in vivo mRNA-LNP exposure

We describe a protocol to study inflammatory responses triggered by the mRNA-lipid nanoparticle (LNP) vaccine formulations in skin, muscle, and lung and the adaptive immune responses induced in the draining lymph nodes. Here, we will present how to deliver these reagents through intradermal, intramu...

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Detalles Bibliográficos
Autores principales: Ndeupen, Sonia, Qin, Zhen, Igyártó, Botond Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127192/
https://www.ncbi.nlm.nih.gov/pubmed/35620070
http://dx.doi.org/10.1016/j.xpro.2022.101350
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author Ndeupen, Sonia
Qin, Zhen
Igyártó, Botond Z.
author_facet Ndeupen, Sonia
Qin, Zhen
Igyártó, Botond Z.
author_sort Ndeupen, Sonia
collection PubMed
description We describe a protocol to study inflammatory responses triggered by the mRNA-lipid nanoparticle (LNP) vaccine formulations in skin, muscle, and lung and the adaptive immune responses induced in the draining lymph nodes. Here, we will present how to deliver these reagents through intradermal, intramuscular, and intranasal routes, generating single-cell suspensions from the inoculated and target organs for downstream analyses. For complete details on the use and execution of this protocol, please refer to Ndeupen et al. (2021) and (2022).
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spelling pubmed-91271922022-05-25 Single-cell suspension preparation from murine organs following in vivo mRNA-LNP exposure Ndeupen, Sonia Qin, Zhen Igyártó, Botond Z. STAR Protoc Protocol We describe a protocol to study inflammatory responses triggered by the mRNA-lipid nanoparticle (LNP) vaccine formulations in skin, muscle, and lung and the adaptive immune responses induced in the draining lymph nodes. Here, we will present how to deliver these reagents through intradermal, intramuscular, and intranasal routes, generating single-cell suspensions from the inoculated and target organs for downstream analyses. For complete details on the use and execution of this protocol, please refer to Ndeupen et al. (2021) and (2022). Elsevier 2022-05-18 /pmc/articles/PMC9127192/ /pubmed/35620070 http://dx.doi.org/10.1016/j.xpro.2022.101350 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ndeupen, Sonia
Qin, Zhen
Igyártó, Botond Z.
Single-cell suspension preparation from murine organs following in vivo mRNA-LNP exposure
title Single-cell suspension preparation from murine organs following in vivo mRNA-LNP exposure
title_full Single-cell suspension preparation from murine organs following in vivo mRNA-LNP exposure
title_fullStr Single-cell suspension preparation from murine organs following in vivo mRNA-LNP exposure
title_full_unstemmed Single-cell suspension preparation from murine organs following in vivo mRNA-LNP exposure
title_short Single-cell suspension preparation from murine organs following in vivo mRNA-LNP exposure
title_sort single-cell suspension preparation from murine organs following in vivo mrna-lnp exposure
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127192/
https://www.ncbi.nlm.nih.gov/pubmed/35620070
http://dx.doi.org/10.1016/j.xpro.2022.101350
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