Protocol for drug screening of patient-derived tumor organoids using high-content fluorescent imaging

High-content imaging of tumor organoids (TOs) treated with therapeutic agents provides detailed cell viability readouts at the organoid level. In contrast, most used protocols provide one number per well. While requiring the use of inverted microscopy with an automated stage, this protocol can provi...

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Detalles Bibliográficos
Autores principales: Larsen, Brian M., Cancino, Andrea, Shaxted, Jenna M., Salahudeen, Ameen A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127194/
https://www.ncbi.nlm.nih.gov/pubmed/35620075
http://dx.doi.org/10.1016/j.xpro.2022.101407

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127194/
https://www.ncbi.nlm.nih.gov/pubmed/35620075
http://dx.doi.org/10.1016/j.xpro.2022.101407

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