Optimized proximity ligation assay (PLA) for detection of RNA-protein complex interactions in cell lines

Conventional proximity ligation assay (PLA) suffers from target specificity issues that curtail their accuracy on interpreting proximal interactions in cell biology. Here, we present a reliable and sensitive approach by including a fluorochrome-labeled mRNA fragment along with biotin-labeled RNA pro...

Descripción completa

Detalles Bibliográficos
Autores principales: George, Jasmine, Mittal, Sonam, Kadamberi, Ishaque P., Pradeep, Sunila, Chaluvally-Raghavan, Pradeep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127197/
https://www.ncbi.nlm.nih.gov/pubmed/35620072
http://dx.doi.org/10.1016/j.xpro.2022.101340

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127197/
https://www.ncbi.nlm.nih.gov/pubmed/35620072
http://dx.doi.org/10.1016/j.xpro.2022.101340

Ejemplares similares