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Optimized proximity ligation assay (PLA) for detection of RNA-protein complex interactions in cell lines

Conventional proximity ligation assay (PLA) suffers from target specificity issues that curtail their accuracy on interpreting proximal interactions in cell biology. Here, we present a reliable and sensitive approach by including a fluorochrome-labeled mRNA fragment along with biotin-labeled RNA pro...

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Detalles Bibliográficos
Autores principales:	George, Jasmine, Mittal, Sonam, Kadamberi, Ishaque P., Pradeep, Sunila, Chaluvally-Raghavan, Pradeep
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127197/ https://www.ncbi.nlm.nih.gov/pubmed/35620072 http://dx.doi.org/10.1016/j.xpro.2022.101340

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