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Targeted ablation of Lgr5-expressing intestinal stem cells in diphtheria toxin receptor-based mouse and organoid models

Intestinal cells marked by Lgr5 function as tissue-resident stem cells that sustain the homeostatic replenishment of the epithelium. By incorporating a diphtheria toxin receptor (DTR) cassette linked to the Lgr5 coding region, native Lgr5-expressing cells are susceptible to ablation upon DT administ...

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Detalles Bibliográficos
Autores principales: Lim, Hui Yi Grace, Yada, Swathi, Barker, Nick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127205/
https://www.ncbi.nlm.nih.gov/pubmed/35620071
http://dx.doi.org/10.1016/j.xpro.2022.101411
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author Lim, Hui Yi Grace
Yada, Swathi
Barker, Nick
author_facet Lim, Hui Yi Grace
Yada, Swathi
Barker, Nick
author_sort Lim, Hui Yi Grace
collection PubMed
description Intestinal cells marked by Lgr5 function as tissue-resident stem cells that sustain the homeostatic replenishment of the epithelium. By incorporating a diphtheria toxin receptor (DTR) cassette linked to the Lgr5 coding region, native Lgr5-expressing cells are susceptible to ablation upon DT administration in vivo. A similar strategy can be used for Lgr5-expressing cells within organoids established from DTR models. Together, these in vivo and in vitro approaches will facilitate dissection of the roles of Lgr5-expressing cells residing in different tissue compartments. For complete details on the use and execution of this protocol, please refer to Tan et al. (2021).
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spelling pubmed-91272052022-05-25 Targeted ablation of Lgr5-expressing intestinal stem cells in diphtheria toxin receptor-based mouse and organoid models Lim, Hui Yi Grace Yada, Swathi Barker, Nick STAR Protoc Protocol Intestinal cells marked by Lgr5 function as tissue-resident stem cells that sustain the homeostatic replenishment of the epithelium. By incorporating a diphtheria toxin receptor (DTR) cassette linked to the Lgr5 coding region, native Lgr5-expressing cells are susceptible to ablation upon DT administration in vivo. A similar strategy can be used for Lgr5-expressing cells within organoids established from DTR models. Together, these in vivo and in vitro approaches will facilitate dissection of the roles of Lgr5-expressing cells residing in different tissue compartments. For complete details on the use and execution of this protocol, please refer to Tan et al. (2021). Elsevier 2022-05-18 /pmc/articles/PMC9127205/ /pubmed/35620071 http://dx.doi.org/10.1016/j.xpro.2022.101411 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lim, Hui Yi Grace
Yada, Swathi
Barker, Nick
Targeted ablation of Lgr5-expressing intestinal stem cells in diphtheria toxin receptor-based mouse and organoid models
title Targeted ablation of Lgr5-expressing intestinal stem cells in diphtheria toxin receptor-based mouse and organoid models
title_full Targeted ablation of Lgr5-expressing intestinal stem cells in diphtheria toxin receptor-based mouse and organoid models
title_fullStr Targeted ablation of Lgr5-expressing intestinal stem cells in diphtheria toxin receptor-based mouse and organoid models
title_full_unstemmed Targeted ablation of Lgr5-expressing intestinal stem cells in diphtheria toxin receptor-based mouse and organoid models
title_short Targeted ablation of Lgr5-expressing intestinal stem cells in diphtheria toxin receptor-based mouse and organoid models
title_sort targeted ablation of lgr5-expressing intestinal stem cells in diphtheria toxin receptor-based mouse and organoid models
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127205/
https://www.ncbi.nlm.nih.gov/pubmed/35620071
http://dx.doi.org/10.1016/j.xpro.2022.101411
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AT barkernick targetedablationoflgr5expressingintestinalstemcellsindiphtheriatoxinreceptorbasedmouseandorganoidmodels