Cargando…

Targeted ablation of Lgr5-expressing intestinal stem cells in diphtheria toxin receptor-based mouse and organoid models

Intestinal cells marked by Lgr5 function as tissue-resident stem cells that sustain the homeostatic replenishment of the epithelium. By incorporating a diphtheria toxin receptor (DTR) cassette linked to the Lgr5 coding region, native Lgr5-expressing cells are susceptible to ablation upon DT administ...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lim, Hui Yi Grace, Yada, Swathi, Barker, Nick
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127205/ https://www.ncbi.nlm.nih.gov/pubmed/35620071 http://dx.doi.org/10.1016/j.xpro.2022.101411

Ejemplares similares

Lgr5(+) intestinal stem cell sorting and organoid culture
por: Liu, Ruixue, et al.
Publicado: (2019)

A permeability assay for mouse intestinal organoids
por: den Daas, Stijn Aaron, et al.
Publicado: (2022)

Neonatal Wnt-dependent Lgr5 positive stem cells are essential for uterine gland development
por: Seishima, Ryo, et al.
Publicado: (2019)

Frizzled7 Functions as a Wnt Receptor in Intestinal Epithelial Lgr5(+) Stem Cells
por: Flanagan, Dustin J., et al.
Publicado: (2015)

PP2A Deficiency Enhances Carcinogenesis of Lgr5(+) Intestinal Stem Cells Both in Organoids and In Vivo
por: Yen, Yu-Ting, et al.
Publicado: (2019)

Establishment and long-term culture of mouse mammary stem cell organoids and breast tumor organoids
por: Cui, Jihong, et al.
Publicado: (2021)

Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids
por: Heinz, Maria C., et al.
Publicado: (2020)

Effects of Various Marine Toxins on the Mouse Intestine Organoid Model
por: Wang, Nengzhuang, et al.
Publicado: (2022)

Quantifying Lgr5-positive stem cell behaviour in the pyloric epithelium
por: Leushacke, Marc, et al.
Publicado: (2016)

Establishment and Culture of Human Intestinal Organoids Derived from Adult Stem Cells
por: Pleguezuelos‐Manzano, Cayetano, et al.
Publicado: (2020)

Epigenetic silencing of Lgr5 induces senescence of intestinal epithelial organoids during the process of aging
por: Uchida, Ryoei, et al.
Publicado: (2018)

Analysis of mouse intestinal organoid culture with conditioned media isolated from mucosal enteric glial cells
por: Baghdadi, Meryem B., et al.
Publicado: (2022)

Identification of diphtheria toxin receptor and a nonproteinous diphtheria toxin-binding molecule in Vero cell membrane
Publicado: (1988)

Heterogeneity of the Level of Activity of Lgr5+ Intestinal Stem Cells
por: Moossavi, Shirin
Publicado: (2014)

Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells
por: Ciarpella, Francesca, et al.
Publicado: (2023)

Monolayer culture of intestinal epithelium sustains Lgr5(+) intestinal stem cells
por: Liu, Yuan, et al.
Publicado: (2018)

Whole-mount staining of mouse colorectal cancer organoids and fibroblast-organoid co-cultures
por: Martinez-Ordoñez, Anxo, et al.
Publicado: (2023)

Publisher Correction: Epigenetic silencing of Lgr5 induces senescence of intestinal epithelial organoids during the process of aging
por: Uchida, Ryoei, et al.
Publicado: (2019)

THE PRODUCTION OF DIPHTHERIA TOXIN
por: Park, W. H., et al.
Publicado: (1896)

ON THE NATURE OF DIPHTHERIA TOXIN
por: Dernby, Karl Gustav
Publicado: (1926)

Diphtheria and Anti-Toxine
por: Crombie, A.
Publicado: (1895)

Diphtheria Anti-Toxin
Publicado: (1898)

The Complexity of Diphtheria Toxin
Publicado: (1914)

Diphtheria and Anti-Toxin
Publicado: (1897)

Anti-Toxine for Diphtheria
Publicado: (1897)

Diphtheria and Anti-Toxin
Publicado: (1896)

LGR5 and LGR6 in stem cell biology and ovarian cancer
por: Schindler, Adam J., et al.
Publicado: (2017)

Analysis of LGR4 Receptor Distribution in Human and Mouse Tissues
por: Yi, Jing, et al.
Publicado: (2013)

LGR5(+) epithelial tumor stem-like cells generate a 3D-organoid model for ameloblastoma
por: Chang, Ting-Han, et al.
Publicado: (2020)

Intestinal organoid co-culture protocol to study cell competition in vitro
por: van Neerven, Sanne M., et al.
Publicado: (2021)

Generation, culture, and stimulation of small intestinal murine organoids in parasitology research
por: Campillo Poveda, Marta, et al.
Publicado: (2023)

Tissue Responses to Shiga Toxin in Human Intestinal Organoids
por: Pradhan, Suman, et al.
Publicado: (2020)

Lgr5(+) intestinal stem cells reside in an unlicensed G(1) phase
por: Carroll, Thomas D., et al.
Publicado: (2018)

LGR5 controls extracellular matrix production by stem cells in the developing intestine
por: Fernandez Vallone, Valeria, et al.
Publicado: (2020)

A brain-sparing diphtheria toxin for chemical genetic ablation of peripheral cell lineages
por: Pereira, Mafalda M. A., et al.
Publicado: (2017)

The Anti-Toxin Treatment of Diphtheria
Publicado: (1894)

Obtaining purified human intestinal epithelia for single-cell analysis and organoid culture
por: Ross, Alexander D.B., et al.
Publicado: (2021)

Differentiation and CRISPR-Cas9-mediated genetic engineering of human intestinal organoids
por: Martinez-Silgado, Adriana, et al.
Publicado: (2022)

Protocol to generate large human intestinal organoids using a rotating bioreactor
por: Takahashi, Junichi, et al.
Publicado: (2023)

BMP restricts stemness of intestinal Lgr5(+) stem cells by directly suppressing their signature genes
por: Qi, Zhen, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_2k8o73rilaksuvthamjpsgfssj