Cell-specific RNA purification to study translatomes of mouse central nervous system

Cell-specific RNA sequencing has revolutionized the study of cell biology. Here, we present a protocol to assess cell-specific translatomes of genetically targeted cell types. We focus on astrocytes and describe RNA purification using RiboTag tools. Unlike single-cell RNA sequencing, this approach a...

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Detalles Bibliográficos
Autores principales: Bravo-Ferrer, Isabel, Khakh, Baljit S., Díaz-Castro, Blanca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127423/
https://www.ncbi.nlm.nih.gov/pubmed/35620074
http://dx.doi.org/10.1016/j.xpro.2022.101397

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127423/
https://www.ncbi.nlm.nih.gov/pubmed/35620074
http://dx.doi.org/10.1016/j.xpro.2022.101397

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