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Isolation of Viable Single Cells With High Yield and Purity Using a Small Amount of Human Kidney Tissue Biopsy

Objective: Establishment of an efficient method of preparing human kidney single cell suspension, using a very small amount of tissue puncture. Methods: Samples of human kidney tissue puncture were cut into pieces, and then 80 μL of the digestive enzyme were added to each punctured tissue to induce...

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Detalles Bibliográficos
Autores principales: Yaigoub, Hasnaa, Tirichen, Hasna, Xin, Xiaohong, Shi, Shuhong, Wu, Changxin, Li, Rongshan, Li, Yafeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9127796/
https://www.ncbi.nlm.nih.gov/pubmed/35620054
http://dx.doi.org/10.3389/fcell.2022.822275