The versatile electric condition in mouse embryos for genome editing using a three-step square-wave pulse electroporator

Technique for Animal Knockout system by Electroporation (TAKE) is a simple and efficient method to generate genetically modified (GM) mice using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems. To reinforce the versatility of electropo...

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Detalles Bibliográficos
Autores principales: Nakano, Kenta, Shimizu, Yukiko, Arai, Tetsuya, Kaneko, Taketo, Okamura, Tadashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2021
Materias:
Original
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130034/
https://www.ncbi.nlm.nih.gov/pubmed/34880157
http://dx.doi.org/10.1538/expanim.21-0130

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130034/
https://www.ncbi.nlm.nih.gov/pubmed/34880157
http://dx.doi.org/10.1538/expanim.21-0130

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