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The versatile electric condition in mouse embryos for genome editing using a three-step square-wave pulse electroporator
Technique for Animal Knockout system by Electroporation (TAKE) is a simple and efficient method to generate genetically modified (GM) mice using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems. To reinforce the versatility of electropo...
Autores principales: | Nakano, Kenta, Shimizu, Yukiko, Arai, Tetsuya, Kaneko, Taketo, Okamura, Tadashi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Association for Laboratory Animal Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9130034/ https://www.ncbi.nlm.nih.gov/pubmed/34880157 http://dx.doi.org/10.1538/expanim.21-0130 |
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