Quantifying telomeric lncRNAs using PNA-labelled RNA-Flow FISH (RNA-Flow)

Here we present a method to detect and quantify long non-coding RNAs, in particular those related to telomeres. By coupling the specificity of a peptide nucleic acid (PNA) probe with flow cytometry we have quantified cellular levels of TERRA and TERC lncRNAs in culture cell lines and PBMCs. This eas...

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Detalles Bibliográficos
Autores principales: González-Vasconcellos, Iria, Cobos-Fernández, María A., Atkinson, Michael J., Fernandez-Piqueras, José, Santos, Javier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132901/
https://www.ncbi.nlm.nih.gov/pubmed/35614335
http://dx.doi.org/10.1038/s42003-022-03452-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132901/
https://www.ncbi.nlm.nih.gov/pubmed/35614335
http://dx.doi.org/10.1038/s42003-022-03452-3

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