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Quantifying telomeric lncRNAs using PNA-labelled RNA-Flow FISH (RNA-Flow)
Here we present a method to detect and quantify long non-coding RNAs, in particular those related to telomeres. By coupling the specificity of a peptide nucleic acid (PNA) probe with flow cytometry we have quantified cellular levels of TERRA and TERC lncRNAs in culture cell lines and PBMCs. This eas...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9132901/ https://www.ncbi.nlm.nih.gov/pubmed/35614335 http://dx.doi.org/10.1038/s42003-022-03452-3 |